Application of 1081-17-0, Chemistry is the science of change. But why do chemical reactions take place? Why do chemicals react with each other? The answer is in thermodynamics and kinetics.In a document type is Article, and a compound is mentioned, 1081-17-0, 1-(4-Methoxyphenyl)-1H-pyrrole-2,5-dione, introducing its new discovery.
Confirming therapeutic target of protopine using immobilized beta2-adrenoceptor coupled with site-directed molecular docking and the target-drug interaction by frontal analysis and injection amount?dependent method
Drug-protein interaction analysis is pregnant in designing new leads during drug discovery. We prepared the stationary phase containing immobilized beta2-adrenoceptor (beta2-AR) by linkage of the receptor on macroporous silica gel surface through N,N?-carbonyldiimidazole method. The stationary phase was applied in identifying antiasthmatic target of protopine guided by the prediction of site-directed molecular docking. Subsequent application of immobilized beta2-AR in exploring the binding of protopine to the receptor was realized by frontal analysis and injection amount?dependent method. The association constants of protopine to beta2-AR by the 2 methods were (1.00?¡À?0.06)?¡Á?105M?1 and (1.52?¡À?0.14)?¡Á?104M?1. The numbers of binding sites were (1.23?¡À?0.07)?¡Á?10?7M and (9.09?¡À?0.06)?¡Á?10?7M, respectively. These results indicated that beta2-AR is the specific target for therapeutic action of protopine in vivo. The target-drug binding occurred on Ser169 in crystal structure of the receptor. Compared with frontal analysis, injection amount?dependent method is advantageous to drug saving, improvement of sampling efficiency, and performing speed. It has grave potential in high-throughput drug-receptor interaction analysis.
Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law.Application of 1081-17-0. In my other articles, you can also check out more blogs about 1081-17-0
Reference£º
Pyrroline – Wikipedia,
1-Pyrroline | C4H7N – PubChem