Chemistry is the experimental science by definition. We want to make observations to prove hypothesis. For this purpose, we perform experiments in the lab. , Application In Synthesis of DL-alpha-Tocopherol, 10191-41-0, Name is DL-alpha-Tocopherol, molecular formula is C29H50O2, belongs to pyrrolines compound. In a document, author is POU, S, introduce the new discover.
THE USE OF FLUOROPHORE-CONTAINING SPIN TRAPS AS POTENTIAL PROBES TO LOCALIZE FREE-RADICALS IN CELLS WITH FLUORESCENCE IMAGING METHODS
Central to the study of free radical processes is the ability to identify and localize their cellular site of formation. Under the best of experimental conditions, spin trapping/ESR spectroscopy can only characterize intracellular production of specific free radicals and confocal microscopy can only localize the site of their formation. In this article, we report on the development of a fluorophore-containing nitrone, alpha-[4-[5-((2-carboxy)phenyl)-5-hydroxy-4-oxo-3-phenyl)-2-pyrrolin-1-yl]phenyl]-N-(tert-butyl)nitrone sodium salt (4).(3) This nitrone (4) reacts with alpha-hydroxyethyl radical with a second order rate constant of 1.7 x 10(5) M(-1) s(-1) to give a characteristic ESR spectrum. However, we were unable to decrease the fluorescence emission, due in part to the small concentration of nitroxide generated from the reaction of alpha-hydroxyethyl radical with nitrone (4). Using the fluorophore-containing nitroxide (7) as a model, we found that only 12% of the nitroxide needs to be reduced to give an almost 400% increase in the fluorescent emission of (7). Our findings suggest new approaches to the development of various fluorophore-containing nitrones that can both characterize specific free radicals and localize their site of intracellular formation.
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Reference:
Pyrroline – Wikipedia,
,1-Pyrroline | C4H7N – PubChem