Synthetic Route of 591-50-4, The transformation of simple hydrocarbons into more complex and valuable products via catalytic C¨CH bond functionalisation has revolutionised modern synthetic chemistry. 591-50-4, Name is Iodobenzene, SMILES is IC1=CC=CC=C1, belongs to pyrrolines compound. In a article, author is Schiemann, Olav, introduce new discover of the category.
Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances
In this protocol, we describe the facile synthesis of the nitroxide spin-label 2,2,5,5-tetramethyl-pyrrolin-1-oxyl-3-acetylene (TPA) and then its coupling to DNA/RNA through Sonogashira cross-coupling during automated solid-phase synthesis. Subsequently, we explain how to perform distance measurements between two such spin-labels on RNA/DNA using the pulsed electron paramagnetic resonance method pulsed electron double resonance (PELDOR). This combination of methods can be used to study global structure elements of oligonucleotides in frozen solution at RNA/DNA amounts of similar to 10 nmol. We especially focus on the Sonogashira cross-coupling step, the advantages of the ACE chemistry together with the appropriate parameters for the RNA synthesizer and on the PELDOR data analysis. This procedure is applicable to RNA/DNA strands of up to similar to 80 bases in length and PELDOR yields reliably spin-spin distances up to similar to 6.5 nm. The synthesis of TPA takes similar to 5 days and spin labeling together with purification similar to 4 days. The PELDOR measurements usually take similar to 16 h and data analysis from an hour up to several days depending on the extent of analysis.
Synthetic Route of 591-50-4, Each elementary reaction can be described in terms of its molecularity, the number of molecules that collide in that step. The slowest step in a reaction mechanism is the rate-determining step.you can also check out more blogs about 591-50-4.
Reference:
Pyrroline – Wikipedia,
,1-Pyrroline | C4H7N – PubChem