In chemical reaction engineering, simulations are useful for investigating and optimizing a particular reaction process or system. 54-47-7, Name is Pyridoxal phosphate, molecular formula is C8H10NO6P. In an article, author is Schiemann, Olav,once mentioned of 54-47-7, Reference of 54-47-7.
Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances
In this protocol, we describe the facile synthesis of the nitroxide spin-label 2,2,5,5-tetramethyl-pyrrolin-1-oxyl-3-acetylene (TPA) and then its coupling to DNA/RNA through Sonogashira cross-coupling during automated solid-phase synthesis. Subsequently, we explain how to perform distance measurements between two such spin-labels on RNA/DNA using the pulsed electron paramagnetic resonance method pulsed electron double resonance (PELDOR). This combination of methods can be used to study global structure elements of oligonucleotides in frozen solution at RNA/DNA amounts of similar to 10 nmol. We especially focus on the Sonogashira cross-coupling step, the advantages of the ACE chemistry together with the appropriate parameters for the RNA synthesizer and on the PELDOR data analysis. This procedure is applicable to RNA/DNA strands of up to similar to 80 bases in length and PELDOR yields reliably spin-spin distances up to similar to 6.5 nm. The synthesis of TPA takes similar to 5 days and spin labeling together with purification similar to 4 days. The PELDOR measurements usually take similar to 16 h and data analysis from an hour up to several days depending on the extent of analysis.
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Reference:
Pyrroline – Wikipedia,
,1-Pyrroline | C4H7N – PubChem